Integrated Lab-on-chip Universal Bio-sample Preparation Module

We propose to develop a self-contained, integrated, disposable universal sampling platform that enables streamlined workflow in the preparation of viral and bacterial sample and obtaining PCR-ready nucleic acids and immuno-reactive content from a variety of sample matrices such as water, whole blood and plasma (virtually any biological matrix). The platform consist a low cost, disposable, microfluidic-based cassette. The cassette accepts a sample of biological fluid and performs lysis; nucleic acid isolation, concentration, as well as protein isolation and purification. The cassette stores on-board all the required buffers and dry reagents, as well as, provides on-chip pumping and flow control. The performance of the module device will be evaluated and tested by identifying the presence of Bacteria E. Coli in spiked samples. The proposed sample preparation system will allow the non-skilled user to process raw samples in a rapid, simple process, enabling push button automated lysis, concentration and purification of isolation of disease specific RNA and DNA, peptides and proteins.